Biosafety Level: 1
6 lyophilized pellets of a single microorganism strain for quality control
Biosafety Level | 1 |
Product Format | LYFO DISK™ |
Test Method | Microbial Identification |
Catalog number | 01026 |
Taxonomy | Bacteria |
Media | Phase 1: ISF (modified Infant Soy Formula) Broth, Phase 2: Tranfer to Sulfite Agar |
Temperature | Phase 1: 55°C, Phase 2: 55°C |
Atmosphere | Phase 1: Anaerobic, Phase 2: Anaerobic |
Growth Time | Phase 1: 48 hours, Phase 2: 48 hours to 7 days |
Additional Tips | Prepare and Use ISF (modified Infant Soy Formula) Broth using the following steps: 1. Fill tubes with 10 ml Infant Soy Formula. Infant Soy Formula may be purchased at a grocery store. 2. Place a four-penny nail in each tube. A four-penny nail is approximately 1.5 inches, or 38 mm, in length. It should contain steel or iron. 3. Sterilize the broth. 4. Inoculate ISF Broth with one LYFO DISK® or KWIK-STIK™. 5. Grow at 55°C in anaerobic conditions for 48 hours. The broth will turn grey, indicating growth. 6. Make two dilutions, 1:10 and 1:100. 7. Sub with a swab to Sulfite Agar. Plate the undiluted sample and the 1:10 and 1:100 dilutions. It is necessary to plate the diluted samples because at higher concentrations the colonies are pin-point which makes colony characteristics difficult to see. Sulfite Agar is used for detecting thermophilic anaerobes which produce sulfite. 8. Incubate the agar in anaerobic environment at 55°C for 48 hours to 7 days. |
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