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EZ-CFU™

EZ-CFU

With EZ-CFU ready-to-use reference cultures, Growth Promotion Testing doesn’t have to be a hassle. Delivering 10-100 CFU per 0.1 ml inoculum, following a simple 1:10 dilution step, each microorganism suspension provides up to 90 tests. These handy kits allow for fast, reliable Growth Promotion Testing. Plus, EZ-CFU microorganisms are just three passages from the reference strains. All in all, EZ-CFU makes meeting Pharmacopeia standards for Growth Promotion Testing easier than ever.

 

 

HIGHLIGHTS

  • Accredited reference material under ISO 17034 standard
  • Delivers 10-100 CFU per 0.1 ml following a 1:10 dilution step
  • 900+ tests per kit, ideal for high volume Growth Promotion Testing
  • Strains are less than or equal to three passages from reference culture – meets USP/Ph. Eur./JP requirements
  • Refrigerated storage is easy and economical
  • Online Certificate of Analysis provides detailed strain information
  • Traceability to reference cultures ensures authenticity
  • Technical Support experts available for guidance
  • Product warranty

Applications and Test Methods

  • Growth Promotion Testing
  • Media Challenge Testing
  • Suitability of Counting Methods
  • Suitability of Sterility Tests
  • Suitability of Tests for Specified Microorganisms
  • Microbial Limits Testing
  • Microbial Enumeration Testing
  • Validation of Neutralization Methods
  • Methods requiring a low CFU concentration

This product is not intended to diagnose, treat, cure or prevent any disease.

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Klebsiella aerogenes derived from ATCC® 13048™
0306C

20 pellets of a microorganism delivering less than 100 CFU per 0.1 mL and 10 vials [2.0 mL ea] of hydrating fluid. Following the dilution step, EZ-CFU™ provides over 90 inocula using the same suspension.

Biosafety Level 1
Product FormatEZ-CFU™
Strain CharacteristicsFormerly Enterobacter aerogenes
Test MethodGrowth Promotion Testing
Catalog number0306
TaxonomyBacteria
Industry TypePharmaceutical

Clostridium sporogenes ATCC® 19404 ™ *
0317C


Biosafety Level: 1


20 pellets of a microorganism delivering less than 100 CFU per 0.1 mL and 10 vials [2.0 mL ea] of hydrating fluid. Following the dilution step, EZ-CFU™ provides over 90 inocula using the same suspension.

Biosafety Level 1
Product FormatEZ-CFU™
Test MethodGrowth Promotion Testing, Sterility Testing, Testing for Specified Microorganisms
WDCM Number00008
Catalog number0317
Standards and GuidelinesEuropean Pharmacopoeia (Ph. Eur.), Japanese Pharmacopoeia (JP), U.S. Pharmacopeia (USP)
TaxonomyBacteria
Industry TypePharmaceutical

Pseudomonas aeruginosa ATCC® 27853 ™ *
0353C


Biosafety Level: 2


20 pellets of a microorganism delivering less than 100 CFU per 0.1 mL and 10 vials [2.0 mL ea] of hydrating fluid. Following the dilution step, EZ-CFU™ provides over 90 inocula using the same suspension.

Biosafety Level 2
Product FormatEZ-CFU™
Strain CharacteristicsMicrobiologics does not recommend the use of this strain for testing Ceftriaxone using the disk diffusion method. We have found that this strain is displaying resistance to Ceftriaxone using this method.

This strain may not give consistent test results for the Ceftriaxone MIC well
Test MethodGrowth Promotion Testing
WDCM Number00025
Catalog number0353
TaxonomyBacteria
Industry TypePharmaceutical

Staphylococcus aureus subsp. aureus ATCC® 25923 ™ *
0360C


Biosafety Level: 2


20 pellets of a microorganism delivering less than 100 CFU per 0.1 mL and 10 vials [2.0 mL ea] of hydrating fluid. Following the dilution step, EZ-CFU™ provides over 90 inocula using the same suspension.

recommended for CAMP Test

Biosafety Level 2
Product FormatEZ-CFU™
Test MethodGrowth Promotion Testing
WDCM Number00034
Catalog number0360
TaxonomyBacteria
Industry TypePharmaceutical

Salmonella enterica subsp. enterica serovar Typhimurium ATCC® 14028 ™
0363C


Biosafety Level: 2


20 pellets of a microorganism delivering less than 100 CFU per 0.1 mL and 10 vials [2.0 mL ea] of hydrating fluid. Following the dilution step, EZ-CFU™ provides over 90 inocula using the same suspension.

Biosafety Level 2
Product FormatEZ-CFU™
Strain CharacteristicsGroup B
You must obtain either VS 16-6 or 16-6a permit from USDA for this material.
Test MethodGrowth Promotion Testing, Testing for Specified Microorganisms
WDCM Number00031
Catalog number0363
Standards and GuidelinesEuropean Pharmacopoeia (Ph. Eur.), Japanese Pharmacopoeia (JP), U.S. Pharmacopeia (USP)
TaxonomyBacteria
Industry TypePharmaceutical

Enterococcus faecalis ATCC® 29212 ™ *
0366C


Biosafety Level: 2


20 pellets of a microorganism delivering less than 100 CFU per 0.1 mL and 10 vials [2.0 mL ea] of hydrating fluid. Following the dilution step, EZ-CFU™ provides over 90 inocula using the same suspension.

Biosafety Level 2
Product FormatEZ-CFU™
Test MethodGrowth Promotion Testing
WDCM Number00087
Catalog number0366
TaxonomyBacteria
Industry TypePharmaceutical

Staphylococcus epidermidis ATCC® 12228 ™ *
0371C


Biosafety Level: 1


20 pellets of a microorganism delivering less than 100 CFU per 0.1 mL and 10 vials [2.0 mL ea] of hydrating fluid. Following the dilution step, EZ-CFU™ provides over 90 inocula using the same suspension.

Biosafety Level 1
Product FormatEZ-CFU™
Test MethodGrowth Promotion Testing
WDCM Number00036
Catalog number0371
TaxonomyBacteria
Industry TypePersonal Care Products/Cosmetics, Pharmaceutical

Streptococcus pyogenes ATCC® 19615 ™ *
0385C


Biosafety Level: 2


20 pellets of a microorganism delivering less than 100 CFU per 0.1 mL and 10 vials [2.0 mL ea] of hydrating fluid. Following the dilution step, EZ-CFU™ provides over 90 inocula using the same suspension.

group A

Biosafety Level 2
Product FormatEZ-CFU™
Strain Characteristicsgroup A
Test MethodGrowth Promotion Testing
Catalog number0385
TaxonomyBacteria
Industry TypePharmaceutical
MediaNon-selective Sheep Blood Agar
Temperature35°C
AtmosphereAerobic
Growth Time24 to 72 hours
Additional TipsNote: Streptococcus will also recover well on Columbia CNA Agar with 5% Sheep Blood.
Note: Growth of some species such as Streptococcus, Arcanobacterium and Trueperella is enhanced by enrichment of the incubation atmosphere with carbon dioxide.
Note: 5% carbon dioxide is recommended for the culture of Streptococcus pneumoniae and other streptococcal species of the viridans group.

Aspergillus brasiliensis ATCC® 16404 ™ *
0392C


Biosafety Level: 1


20 pellets of a microorganism delivering less than 100 CFU per 0.1 mL and 10 vials [2.0 mL ea] of hydrating fluid. Following the dilution step, EZ-CFU™ provides over 90 inocula using the same suspension.

fungus

Biosafety Level 1
Product FormatEZ-CFU™
Test MethodGrowth Promotion Testing, Pharmaceutical Microbial Enumeration Testing, Sterility Testing
WDCM Number00053
Catalog number0392
Standards and GuidelinesEuropean Pharmacopoeia (Ph. Eur.), Japanese Pharmacopoeia (JP), U.S. Pharmacopeia (USP)
TaxonomyFungi
Industry TypeMedical Devices, Nutraceutical/Dietary Supplements, Personal Care Products/Cosmetics, Pharmaceutical

Salmonella enterica subsp. enterica serovar Typhimurium ATCC® 13311 ™
0421C


Biosafety Level: 2


20 pellets of a microorganism delivering less than 100 CFU per 0.1 mL and 10 vials [2.0 mL ea] of hydrating fluid. Following the dilution step, EZ-CFU™ provides over 90 inocula using the same suspension.

Biosafety Level 2
Product FormatEZ-CFU™
Test MethodGrowth Promotion Testing
WDCM Number00121
Catalog number0421
Standards and GuidelinesU.S. Pharmacopeia (USP) - Dietary Supplements
TaxonomyBacteria
Industry TypeNutraceutical/Dietary Supplements, Pharmaceutical

Candida albicans ATCC® 10231 ™ *
0443C


Biosafety Level: 1


20 pellets of a microorganism delivering less than 100 CFU per 0.1 mL and 10 vials [2.0 mL ea] of hydrating fluid. Following the dilution step, EZ-CFU™ provides over 90 inocula using the same suspension.

Biosafety Level 1
Product FormatEZ-CFU™
Strain CharacteristicsYeast cells
Test MethodGrowth Promotion Testing, Pharmaceutical Microbial Enumeration Testing, Sterility Testing, Testing for Specified Microorganisms
WDCM Number00054
Catalog number0443
Standards and GuidelinesEuropean Pharmacopoeia (Ph. Eur.), Japanese Pharmacopoeia (JP), U.S. Pharmacopeia (USP)
TaxonomyFungi
Industry TypeMedical Devices, Nutraceutical/Dietary Supplements, Personal Care Products/Cosmetics, Pharmaceutical

Escherichia coli ATCC® 8739 ™ *
0483C


Biosafety Level: 1


20 pellets of a microorganism delivering less than 100 CFU per 0.1 mL and 10 vials [2.0 mL ea] of hydrating fluid. Following the dilution step, EZ-CFU™ provides over 90 inocula using the same suspension.

Biosafety Level 1
Product FormatEZ-CFU™
Test MethodGrowth Promotion Testing, Sterility Testing, Testing for Specified Microorganisms
WDCM Number00012
Catalog number0483
Standards and GuidelinesEuropean Pharmacopoeia (Ph. Eur.), Japanese Pharmacopoeia (JP), U.S. Pharmacopeia (USP), U.S. Pharmacopeia (USP) - Dietary Supplements
TaxonomyBacteria
Industry TypeMedical Devices, Nutraceutical/Dietary Supplements, Personal Care Products/Cosmetics, Pharmaceutical

Pseudomonas paraeruginosa derived from ATCC® 9027™
0484C


Biosafety Level: 2

20 pellets of a microorganism delivering less than 100 CFU per 0.1 mL and 10 vials [2.0 mL ea] of hydrating fluid. Following the dilution step, EZ-CFU™ provides over 90 inocula using the same suspension.

Biosafety Level 2
Product FormatEZ-CFU™
Test MethodGrowth Promotion Testing, Pharmaceutical Microbial Enumeration Testing, Sterility Testing, Testing for Specified Microorganisms
WDCM Number00026
Catalog number0484
Standards and GuidelinesEuropean Pharmacopoeia (Ph. Eur.), Japanese Pharmacopoeia (JP), U.S. Pharmacopeia (USP), U.S. Pharmacopeia (USP) - Dietary Supplements
TaxonomyBacteria
Industry TypeMedical Devices, Nutraceutical/Dietary Supplements, Personal Care Products/Cosmetics, Pharmaceutical

Staphylococcus aureus subsp. aureus ATCC® 6538 ™ *
0485C


Biosafety Level: 2


20 pellets of a microorganism delivering less than 100 CFU per 0.1 mL and 10 vials [2.0 mL ea] of hydrating fluid. Following the dilution step, EZ-CFU™ provides over 90 inocula using the same suspension.

Biosafety Level 2
Product FormatEZ-CFU™
Test MethodGrowth Promotion Testing, Pharmaceutical Microbial Enumeration Testing, Sterility Testing, Testing for Specified Microorganisms
WDCM Number00032
Catalog number0485
Standards and GuidelinesEuropean Pharmacopoeia (Ph. Eur.), Japanese Pharmacopoeia (JP), U.S. Pharmacopeia (USP), U.S. Pharmacopeia (USP) - Dietary Supplements
TaxonomyBacteria
Industry TypeMedical Devices, Nutraceutical/Dietary Supplements, Personal Care Products/Cosmetics, Pharmaceutical

Bacillus spizizenii derived from ATCC® 6633™
0486C


Biosafety Level: 1


20 pellets of a microorganism delivering less than 100 CFU per 0.1 mL and 10 vials [2.0 mL ea] of hydrating fluid. Following the dilution step, EZ-CFU™ provides over 90 inocula using the same suspension.

Biosafety Level 1
Product FormatEZ-CFU™
Test MethodGrowth Promotion Testing, Pharmaceutical Microbial Enumeration Testing, Sterility Testing
Catalog number0486
Standards and GuidelinesEuropean Pharmacopoeia (Ph. Eur.), Japanese Pharmacopoeia (JP), U.S. Pharmacopeia (USP), U.S. Pharmacopeia (USP) - Dietary Supplements
TaxonomyBacteria
Industry TypeNutraceutical/Dietary Supplements, Pharmaceutical

Clostridium sporogenes ATCC® 11437 ™ *
0487C


Biosafety Level: 1


20 pellets of a microorganism delivering less than 100 CFU per 0.1 mL and 10 vials [2.0 mL ea] of hydrating fluid. Following the dilution step, EZ-CFU™ provides over 90 inocula using the same suspension.

Biosafety Level 1
Product FormatEZ-CFU™
Test MethodGrowth Promotion Testing, Sterility Testing, Testing for Specified Microorganisms
Catalog number0487
Standards and GuidelinesEuropean Pharmacopoeia (Ph. Eur.), Japanese Pharmacopoeia (JP), U.S. Pharmacopeia (USP)
TaxonomyBacteria
Industry TypePharmaceutical

Pseudomonas protegens  ATCC® 17386™
0524C


Biosafety Level: 1


20 pellets of a microorganism delivering less than 100 CFU per 0.1 mL and 10 vials [2.0 mL ea] of hydrating fluid. Following the dilution step, EZ-CFU™ provides over 90 inocula using the same suspension.

Formerly Pseudomonas fluorescens; biotype G

Biosafety Level 1
Product FormatEZ-CFU™
Strain CharacteristicsFormerly Pseudomonas fluorescens
biotype G
Test MethodGrowth Promotion Testing
Catalog number0524
Standards and GuidelinesJapanese Pharmacopoeia (JP)
TaxonomyBacteria
Industry TypePharmaceutical

Listeria monocytogenes ATCC® 19115 ™ *
0687C

20 pellets of a microorganism delivering less than 100 CFU per 0.1 mL and 10 vials [2.0 mL ea] of hydrating fluid. Following the dilution step, EZ-CFU™ provides over 90 inocula using the same suspension.

Biosafety Level 2
Product FormatEZ-CFU™
Strain Characteristicsserotype 4b
Test MethodGrowth Promotion Testing
Catalog number0687
TaxonomyBacteria

Kocuria rhizophila ATCC® 9341 ™ *
0688C


Biosafety Level: 1


20 pellets of a microorganism delivering less than 100 CFU per 0.1 mL and 10 vials [2.0 mL ea] of hydrating fluid. Following the dilution step, EZ-CFU™ provides over 90 inocula using the same suspension.

Biosafety Level 1
Product FormatEZ-CFU™
Test MethodGrowth Promotion Testing, Sterility Testing
Catalog number0688
Standards and GuidelinesU.S. Pharmacopeia (USP)
TaxonomyBacteria
Industry TypePharmaceutical

Lactobacillus fermentum ATCC® 9338 ™ *
0813C


Biosafety Level: 1


20 pellets of a microorganism delivering less than 100 CFU per 0.1 mL and 10 vials [2.0 mL ea] of hydrating fluid. Following the dilution step, EZ-CFU™ provides over 90 inocula using the same suspension.

Biosafety Level 1
Product FormatEZ-CFU™
Test MethodGrowth Promotion Testing
Catalog number0813
TaxonomyBacteria
Industry TypePharmaceutical
MediaPhase 1: MRS (Man, Rogosa, Sharpe) Broth. Phase 2: Transfer to Columbia CNA with Sheep Blood or Tryptic Soy Agar with Sheep Blood
TemperaturePhase 1: 35°C Phase 2: 35°C
AtmospherePhase 1: Aerobic. Phase 2: 5 to 7% Carbon Dioxide
Growth TimePhase 1: 48 hours. Phase 2: 48 hours
Additional TipsNote: For Phase 1, the primary growth medium is MRS (Man, Rogosa, Sharpe) Broth. Incubate at 35°C in aerobic atmosphere for 48 hours. For Phase 2, transfer to either Columbia CNA with Sheep Blood or Tryptic Soy Agar with Sheep Blood. Incubate at 35°C in 5 to 7% carbon dioxide for 48 hours.

Staphylococcus aureus subsp. aureus ATCC® 6538P ™ *
0827C


Biosafety Level: 2


20 pellets of a microorganism delivering less than 100 CFU per 0.1 mL and 10 vials [2.0 mL ea] of hydrating fluid. Following the dilution step, EZ-CFU™ provides over 90 inocula using the same suspension.

Biosafety Level 2
Product FormatEZ-CFU™
Test MethodGrowth Promotion Testing
WDCM Number00033
Catalog number0827
TaxonomyBacteria
Industry TypePharmaceutical

Geobacillus stearothermophilus ATCC® 7953 ™ *
0871C


Biosafety Level: 1


20 pellets of a microorganism delivering less than 100 CFU per 0.1 mL and 10 vials [2.0 mL ea] of hydrating fluid. Following the dilution step, EZ-CFU™ provides over 90 inocula using the same suspension.

Biosafety Level 1
Product FormatEZ-CFU™
Strain CharacteristicsBased on in-house studies, Microbiologics has found that G. stearothermophilus can produce significantly different results on different media brands.
Test MethodGrowth Promotion Testing
Catalog number0871
TaxonomyBacteria
Industry TypePharmaceutical

Geobacillus stearothermophilus ATCC® 12980 ™ *
0872C


Biosafety Level: 1


20 pellets of a microorganism delivering 10 -20 pellets of a microorganism delivering less than 100 CFU per 0.1 mL and 10 vials [2.0 mL ea] of hydrating fluid. Following the dilution step, EZ-CFU™ provides over 90 inocula using the same suspension.

Biosafety Level 1
Product FormatEZ-CFU™
Strain CharacteristicsBased on in-house studies, Microbiologics has found that G. stearothermophilus can produce significantly different results on different media brands.
Test MethodGrowth Promotion Testing
Catalog number0872
TaxonomyBacteria

Salmonella enterica subsp. enterica NCTC 6017
0890C


Biosafety Level: 2


20 pellets of a microorganism delivering less than 100 CFU per 0.1 mL and 10 vials [2.0 mL ea] of hydrating fluid. Following the dilution step, EZ-CFU™ provides over 90 inocula using the same suspension.

serovar Abony

Biosafety Level 2
Product FormatEZ-CFU™
Strain Characteristicsserovar Abony
Test MethodGrowth Promotion Testing, Testing for Specified Microorganisms
WDCM Number00029
Catalog number0890
Standards and GuidelinesEuropean Pharmacopoeia (Ph. Eur.), Japanese Pharmacopoeia (JP), U.S. Pharmacopeia (USP)
TaxonomyBacteria
Industry TypePharmaceutical

Salmonella enterica subsp. enterica serovar Choleraesuis ATCC® 10708 ™
0902C


Biosafety Level: 2


20 pellets of a microorganism delivering less than 100 CFU per 0.1 mL and 10 vials [2.0 mL ea] of hydrating fluid. Following the dilution step, EZ-CFU™ provides over 90 inocula using the same suspension.

H2S negative

Biosafety Level 2
Product FormatEZ-CFU™
Strain CharacteristicsGroup C1
H2S negative
Test MethodGrowth Promotion Testing
Catalog number0902
TaxonomyBacteria
Industry TypePharmaceutical

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